The importance of negative matrices

 

In molecular diagnostics, accuracy is not only about detecting positives but also about confidently confirming negatives. Undetected contamination, matrix effects, or improper samples can compromise even the most robust workflows.
 

That is why negative controls are essential. They verify assay specificity, detect contamination or cross-reactivity, and effectively validate the entire workflow, from extraction to amplification.

Información de producto

Vircell’s AMPLIRUN® Negative Matrices are designed to provide reliable, ready-to-use solutions that strengthen your molecular testing quality:

  • Broadly pre-screened
    Tested against a wide panel of microorganisms (up to 33 viruses, bacteria and fungi tested) to ensure a true negative background.
  • Real clinical matrix that mimics human specimens for realistic validation.
  • Lyophilized to save costs in logistics and reduce variability.
Kit AMPLIRUN TOTAL

PRODUCTS

RESPIRATORY SWAB MATRIX NEGATIVE CONTROL

RUO
Total negative control in a matrix that mimics a human respiratory specimen for monitoring the entire process: extraction, amplification, and detection. Tested negative for more than 30 pathogens associated with respiratory infections.
Reference MC110
Pack size 10 vials
Volume 500 µl

SPUTUM NEGATIVE CONTROL

RUO
A negative control matrix that mimics a human sputum sample, used to monitor the entire process: extraction, amplification and detection. It contains A549 epithelial cells (20,000 cells/mL) from human lung carcinoma. Tested negative for more than 30 pathogens.
Reference MC128
Pack size 10 vials
Volume 500 µl

STOOL MATRIX NEGATIVE CONTROL

RUO
A negative control matrix that mimics a human stool sample, used to monitor the entire process: extraction, amplification and detection. It contains human epithelial cells diluted in accordance with the Bristol Stool Scale to replicate the actual consistency of the sample. Tested negative for more than 10 pathogens.
Reference MC107
Pack size 10 vials
Volume 500 µl

MORE RESOURCES

Blog
BLOG

The latest insights, news, and research articles from our scientific team.

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Polymicrobial Vaginal Infections: why third-party controls matter in molecular testing
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Polymicrobial Vaginal Infections: why third-party controls matter in molecular testing

Understanding the complexity of vaginal infectionsVaginal infections are among the most frequent reasons for gynecological consultation, but their dia...

Bibliography
BIBLIOGRAPHY

Scientific bibliography and references related to this product.

Forrest-Hammond R, Kopec A, Alcolea-Medina A, Batra R, Brown K, Enne V, et al. Implementation of rapid pan-microbial metagenomics in paediatric intensive care: a single-centre, prospective, observational study. Lancet Microbe. 2026 Oct 15:101461.
Hammond R, Kopec A, Alcolea-Medina A, Batra R, Brown K, Enne V, et al. Implementing rapid pan-microbial metagenomics in paediatric intensive care. medRxiv. 2025.
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